|
(Last modification:
21. Mar. 2008)
Methionine
Synthase
Return to citation LOCUS CRMETS 2602 bp mRNA PLN 06-JUL-1995
DEFINITION C.roseus MetE mRNA for methionine synthase.
ACCESSION X83499
NID g886470
VERSION X83499.1 GI:886470
KEYWORDS methionine synthase.
SOURCE Madagascar periwinkle.
ORGANISM Catharanthus roseus
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons;
Asteridae; Gentiananae; Gentianales; Apocynaceae; Catharanthus.
REFERENCE 1 (bases 1 to 2602)
AUTHORS Eichel,J., Gonzalez,J.C., Hotze,M., Matthews,R.G. and Schroeder,J.
TITLE Vitamin-B12-independent methionine synthase from a higher plant
(Catharanthus roseus). Molecular characterization, regulation,
heterologous expression, and enzyme properties
JOURNAL Eur. J. Biochem. 230 (3), 1053-1058 (1995)
MEDLINE 95324563
REFERENCE 2 (bases 1 to 2602)
AUTHORS Schroeder,J.
TITLE Direct Submission
JOURNAL Submitted (14-DEC-1994) J. Schroeder, University of Freiburg,
Inst.f. Biologie II, Schaenzlestr. 1, D- 79104 Freiburg, FRG
FEATURES Location/Qualifiers
source 1..2602
/organism="Catharanthus roseus"
/db_xref="taxon:4058"
gene 51..2348
/gene="metE"
CDS 51..2348
/gene="metE"
/EC_number="2.1.1.14"
/function="L-homocysteine + methyl-tetrahydrofolate-Glu3
-> L-methionine"
/note="Vitamin B12-independent transfer of methyl groups
from polyglutamated methyl-tetrahydrofolates to
L-homocysteine"
/citation=[1]
/codon_start=1
/evidence=experimental
/product="methionine synthase"
/protein_id="CAA58474.1"
/db_xref="PID:g886471"
/db_xref="GI:886471"
/db_xref="SPTREMBL:Q42699"
/translation="MASHIVGYPRMGPKRELKFALESFWDKKSSAEDLQKVAADLRSS
IWKQMADAGIKYIPSNTFSYYDQVLDTATMLGAVPPRYNFAGGEIGFDTYFSMARGNA
SVPAMEMTKWFDTNYHYIVPELGPEVNFSYASHKAVNEYKEAKELGVDTVPVLVGPVT
FLLLSKPAKGVEKTFPLLSLLDKILPVYKEVIGELKAAGASWIQFDEPTLVLDLESHQ
LEAFTKAYSELESTLSGLNVIVETYFADIPAETYKILTALKGVTGFGFDLVRGAKTLD
LIKGGFPSGKYLFAGVVDGRNIWANDLAASLSTLQSLEGIVGKDKLVVSTSCSLLHTA
VDLVNEPKLDKEIKSWLAFAAQKVVEVNALAKALAGEKDEAFFSENAAAQASRKSSPR
VTNQAVQKAAAALRGSDHRRATTVSARLDAQQKKLNLPVLPTTTIGSFPQTLELRRVR
REYKAKKISEDDYVKAIKEEISKVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFA
FTANGWVQSYGSRCVKPPIIYGDVSRPNPMTVFWSQTAQSMTKRPMKGMLTGPVTILN
WSFVRNDQPRFETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKAEHAFYL
DWAVHSFRITNLPLQDTTQIHTHMCYSNFNDIIHSIIDMDADVMTIENSRSSEKLLSV
FREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLDTNILWVNPDCGLKTRKY
AEVKPALENMVSAAKLIRTQLASAK"
Return to citation
BASE COUNT 669 a 583 c 601 g 749 t
ORIGIN
1 ctcggaacca gtctctgtga atccccggtt ccatctcttc ctaaagaaaa atggcttccc
61 acattgttgg ttaccctcgt atgggcccaa agagagaact taaatttgca ttggaatctt
121 tctgggataa aaagagctct gctgaggatt tgcagaaggt ggctgctgac ctcaggtcct
181 ccatctggaa gcagatggcc gatgctggca tcaagtacat tcccagcaac accttctctt
241 actatgatca agtactcgat accgctacaa tgcttggagc tgtgccccct agatacaact
301 ttgctggtgg tgagattgga tttgatacat acttctccat ggctagagga aatgcctctg
361 tacctgctat ggaaatgacc aagtggtttg ataccaacta ccactacatt gttcctgaat
421 tgggacctga agtcaacttt tcttatgctt cccacaaagc agtgaatgaa tacaaggagg
481 ctaaggagct tggtgtagac actgttccag tccttgttgg acctgtcact ttcttgttgc
541 tatctaagcc tgccaagggt gttgagaaaa ctttccccct cctctccctt ctagacaaaa
601 ttcttccagt ctacaaggaa gtaattggtg agctgaaggc agctggtgct tcgtggattc
661 agtttgatga gcccaccttg gtgttggatc ttgaatctca ccagttggaa gcattcacca
721 aggcttactc tgagttggag tcaacactat ctggcctcaa tgttattgtt gagacatatt
781 ttgctgatat tcctgcagag acatacaaaa tcctcactgc tttgaagggg gttacaggtt
841 ttggctttga ccttgttcgt ggagctaaaa ctcttgactt gatcaagggt ggtttcccat
901 ctggcaagta tttgtttgct ggagtcgttg atggaaggaa catctgggcc aatgatcttg
961 ctgcatctct cagcaccttg caatcacttg agggcattgt tggaaaagac aagcttgttg
1021 tttccacctc ctgctcactt ctccacactg ctgttgattt ggttaatgag cctaaattgg
1081 acaaagaaat caaatcttgg cttgcatttg ctgcccaaaa ggttgttgaa gttaatgctt
1141 tggccaaggc attagctggt gagaaggatg aggcgttctt ctccgagaat gctgcagctc
1201 aggcttccag gaagtcctct ccaagggtga ctaatcaagc tgtgcaaaag gctgctgctg
1261 ctttgagagg ttctgaccac cgccgtgcta ccactgttag tgctagactt gatgctcagc
1321 agaagaagct aaaccttcca gttcttccta ccaccaccat tggatccttc ccccagacat
1381 tagagcttag gagagttcgc cgtgagtaca aggccaagaa gatttctgag gatgattatg
1441 ttaaggccat caaggaggaa atcagcaagg ttgtcaagct ccaagaagag cttgacattg
1501 atgttttggt tcatggtgag ccagagagaa acgatatggt tgagtacttc ggagagcagc
1561 tgtcaggttt tgcctttact gctaatggat gggtccaatc ctatggatct cgatgtgtga
1621 agccaccaat catctatggt gatgtgagtc gcccaaatcc catgactgtc ttctggtccc
1681 aaacagctca gagcatgacc aagcgcccaa tgaagggaat gcttactggc cctgttacaa
1741 tcctcaattg gtcttttgtc aggaatgatc agccaagatt tgaaacttgc taccagattg
1801 ccttggccat caaggatgaa gtggaagatc ttgagaaggc aggaatcaat gtcattcaaa
1861 ttgatgaagc tgctttgaga gaagggttgc cactaaggaa ggccgaacac gctttctact
1921 tggactgggc tgtccactcc ttcagaatta caaatcttcc tcttcaggac actacccaga
1981 tccacactca catgtgctac tccaacttca acgatatcat tcactctatc atcgacatgg
2041 atgctgatgt gatgaccatt gagaactcca gatctagtga gaagctcctt tctgtgttcc
2101 gtgagggagt gaagtacgga gctggaattg gccctggtgt ctacgatatc cattccccaa
2161 gaataccatc aacagaggag attgccgaca gaatcaacaa gatgcttgca gtgttggaca
2221 ccaacatctt gtgggttaac cccgactgtg gtctcaagac ccgcaagtac gcagaagtga
2281 aacctgccct tgaaaacatg gtctctgctg ccaagctcat ccgcacacag ttggccagtg
2341 ccaagtgaag aaggaattga gtatggaaaa ctcgaaaatt cattgttctt tttttttaca
2401 aactttacgg gaataccctc ttttgattca ataactgtgg tctgttatga aatattattg
2461 ttagtcaata ggtgtagttt tgcccattgg gtattttgtt ttggtttttc ttttaatctc
2521 tctgtattcc tgagggattt tcgccttcaa ttcatttgaa aggcttcacc ctgattttca
2581 tgtatggaaa agaaatggaa at
// |
